12/9/2023 0 Comments Nucleoplasmin![]() ( C) Glycerol density gradient of HeLa cell nuclear extracts. Images were observed under confocal microscopy. HeLa cells were subjected to immunofluorescence analysis using anti-NPM1 and anti-NPM3 antibodies. ( B) Localization of endogenous NPM1 and NPM3 in HeLa cells. EF, EF–NPM1, EF–NPM2 and EF–NPM3 were transiently expressed in HeLa cells and the localization of EGFP proteins was examined by fluorescent microscopy. ( A) Localization of EGFP-tagged NPM proteins. Our results proposed a novel mechanism whereby three NPM proteins cooperatively regulate chromatin disassembly and assembly in the early embryo and in somatic cells.Īssociation between human NPM proteins. NPM3 also suppressed the RNA-binding activity of NPM1, which enhanced the nucleoplasm-nucleolus shuttling of NPM1 in somatic cell nuclei. Furthermore, the oligomer formation with NPM1 elicited NPM3 nucleosome assembly and sperm chromatin decondensation activity. Maximal sperm chromatin remodeling activity of NPM2 required the inhibition of its non-specific nucleic acid-binding activity by phosphorylation. NPM1 and NPM2 mediated nucleosome assembly independently of other NPM proteins, whereas the function of NPM3 was largely dependent on formation of a complex with NPM1. We characterized the sperm chromatin decondensation and nucleosome assembly activities of three human NPM proteins. In mammals, three nucleoplasmin/nucleophosmin (NPM) family proteins-NPM1, NPM2 and NPM3-expressed in oocytes are presumed to cooperatively regulate sperm chromatin remodeling. This reaction involves the removal of sperm-specific basic proteins and chromatin assembly with histones. Sperm chromatin remodeling after oocyte entry is the essential step that initiates embryogenesis. ![]()
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12/9/2023 0 Comments Tuxguitar mac 64![]() ![]() Supported but MIDI playback will only recognize the first two alternative endings, the second of which must be located above the measure that directly follows the closed repeat in order for it to be associated with the first alternative ending and the repeat in question.Ĭustom Number of Tunings for a Track's strings Supported but limited to either open or closed barline repeats. Supported but limited to two voices per track. Supported, can be displayed by clicking View > Multitrack. Limited support (the program doesn't differentiate between the two). Supported in the form of hammer-ons/pull-offs. Limited to a duration of at most 1/8 and at least 1/32 of a whole note. Limited to a duration ranging from 1/64 to 1/16 of a whole note. Only natural, artificial, tapped, pinched, or semi harmonic. The default skin for version 1.0 is Lavender. Simple, elegant theme with lavender color.Ī more serious theme, where blue dominates. TuxGuitar offers a set of three default skins that its users can choose from. The program is written in the Java programming language and is released under the LGPL-2.1-only license. TuxGuitar's mascot and namesake is Tux, the penguin mascot of many games and programs originally designed for Linux. In addition, TuxGuitar's tablature and staff interfaces function as basic MIDI editors. Once you have familiarized yourself with it, everything will be a piece-of-cake.TuxGuitar is a free and open-source tablature editor, which includes features such as tablature editing, score editing, and import and export of Guitar Pro gp3, gp4, and gp5 files. If you're a beginner user of this app, you should make use of its help section to quickly grasp its whole operation. In spite of its quite confusing first impression, TuxGuitar truly deserves a chance from all music composers especially for its nicely-designed interface that features many interesting tools, which can greatly help improve your music composition. Its built-in player can go through the entire sheet and provide an accurate rendition. While you are working on your current composition you can listen to it and modify the areas you want to improve. Essentially, all the markings in a regular musical sheet are also accessible in this app. If you wish to increase or decrease the tone of a certain note, you can possibly do it here. You'll also be able to select the note value you prefer either whole, half, quarter, eighth, sixteenth, thirty-second, or sixty-fourth.Īt the same time, it allows you to insert a time signature, set the music tempo, and choose a clef. Your attention will surely focus on your on-going composition as it displays an empty music sheet that you must fill in with notes and rhythms. However, its entire operation will still be a challenge, especially for beginners. How do you use TuxGuitar?Īs stated, this music companion app provides a clean interface with a layout that is intuitive-designed. On top of that, it provides import and export support for GP3, GP4, and GP5 file formats. What's more, it also enables you to play the songs you compose, using one of the many instruments included in it. Besides composing on the tablature, you can also use a virtual guitar fretboard or piano keyboard to make composition even easier. ![]() 12/9/2023 0 Comments Kamen rider revice episode 1 sub![]() ![]() But here’s the thing, Bujin sword has only shown higher performance and power, along with teleportation as it’s base abilities. The Bujin sword form easily no caps Buffa, and Daichi, along with several other high level opponents like Gazer, possibly even Kekera and Beroba’s new forms. But he didn’t take into account of Tsumuri abilities to make things into existence. Daichi estimated that he could take on a heartbroken Keiwa with ease, even with the twin set buckle (jet cannon). Keiwa has matured greatly, but there was one new variable, Tsumuri. This is why he didn’t reveal himself until he was sure he could mentally destroy Keiwa, cause Geats could see through everything that Daichi can do. But with Keiwa he sees him as a non entity to be easily dealt with. You have to remember that Daichi has always been cautious of Geats. * Kamen Rider Ginpen– the Penguin Rider is the oldest participant to date a company executive named Takato Taira with a link to Kamen Rider Tycoon. Ginpen’s actor is Tomoharu Hasegawa. Shiro is portrayed by tokusatsu veteran Yamato Kaneshiro, best known as Nossan/Kyoryu Blue in Zyuden Sentai Kyoryuger. * Kamen Rider Shiro– the Polar Bear Rider with the civilian identity of Takeshi Gotokuji is said to be a speedy warrior with a strong sense of justice. Dapan is portrayed by Ryunosuke Miyamoto. * Kamen Rider Dapan– the Panda Rider- named Kanato Sumida is a Rider with a cute face covering his scary behavior. * Kamen Rider Merry– the Sheep Rider whose real name is Morio Koganaya. Merry is described as “clumsy, cunning & a little annoying.” Merry is played by Koji Abe. Toei has revealed three more members of the DESIRE GRAND PRIX in Kamen Rider Geats! Advance screenings of Kamen Rider Revice: Battle Familia revealed a Septempremiere date for Geats along with a short story teaser.Ī press conference was held on August 7, 2022, revealing the main cast members as well as the theme song artists. On June 26, 2022, the copyright trademark updated to reveal the official title card of the show. Toei Company confirmed and filed the trademark for Geats on May 31, 2022. The series catchphrase is This game ― The winner of the end is me ( このゲーム―最後に勝つは 俺 オレだ, Kono Gēmu ― Saigo ni Katsu wa Ore da) The series is scheduled to premiere on September 4, 2022, joining Avataro Sentai Donbrothers in the Super Hero Time lineup after the finale of Kamen Rider Revice. It is the fourth series to debut in the Reiwa Era and the thirty-third overall. This special takes place sometime after the events of Episode 21 as Hiromi returns home after being defeated by Orteca, but before the start of the events of Episode 29 as Ikki and Daiji mention they had visited Hiromi's family home in said episode with scenes from this episode being shown via a flashback.Kamen Rider Geats is an upcoming Japanese Tokusatsu drama in Toei Company’s Kamen Rider Series. What is he feeling in his chest that walks on the edge of despair? Continuity and Placement but he's still alive and returned to his hometown, drained mentally and physically. Hiromi Kadota, the commander of Fenix, is a man who put his life on the line to protect the world and fell off of a cliff from a fierce battle. ![]() ![]() In the special, Hiromi returns to his hometown to recover after his spirit is broken following his fierce battle with Orteca. Kamen Rider Revice Original Spin-Off Drama: DEAR GAGA (仮面ライダーリバイスオリジナルスピンオフドラマ: DEAR GAGAヂーアガガ, Kamen Raidā Ribaisu Orijinaru Supin'ofu Dorama Djīa Gaga) is a two-episode Blu-Ray exclusive miniseries set within the continuity of Kamen Rider Revice. Kamen Rider Revice Original Spin-Off Drama: DEAR GAGA Full English Sub - TokuFun ![]() ![]() ![]() Only 12% of law enforcement officers in California have completed the state-certified training course, according to data from the Commission on Peace Officers Standards and Training. The training of officers on the new law is highly inconsistent across the state, with some agencies enrolling them in a two-hour state-certified course, others creating their own training and some departments just handing officers a memo and a 14-minute video.In the 15 years before 2020, only six California officers were criminally charged for killing on the job, according to data compiled by a criminal justice professor at Bowling Green State University in Ohio. Five officers in California have been criminally charged for on-duty deaths, though two of them (in San Francisco and Contra Costa counties) are being prosecuted for shootings that happened before the new law took effect in another case, the Stanislaus County district attorney won’t say if her decision is based on the new law.The number of fatal police shootings in California rose: from 135 in 2019 to 148 in 2020, according to data tracked by the Washington Post.Nor has the training law led to widespread, state-certified instruction for officers on the new standard for using deadly force.ĬalMatters analyzed data on deadly police shootings, officers charged with crimes for on-duty deaths, and use-of-force training of officers since the law took effect. ![]() How judges and juries apply it in these cases could shape its power in the years to come.īut beyond these two cases, the available evidence so far suggests that the new law has not been as transformative as supporters hoped when they pushed the so-called Act to Save Lives through the Legislature. Prosecutors in both cases cited the law in their decisions to file charges. Addie Kitchen, Steven Taylor’s grandmother, speaks to the crowd at a celebration of life held for Taylor on the one-year anniversary of his death, at the San Leandro Marina on April 18, 2021. Nearly a year-and-a-half since the laws took effect, the two criminal cases - a San Diego sheriff’s deputy charged with second-degree murder and a San Leandro police officer charged with manslaughter - are the most concrete signs that one of the nation’s strictest use-of-force laws is having an impact. ![]() Law enforcement groups put their weight behind companion legislation that was supposed to improve how officers are trained. ![]() So they championed the new law as a way to reduce police shootings and hold officers accountable when they unnecessarily take a life. Passed in 2019 - a year before the killing of George Floyd sparked nationwide protests over racism and police violence - California’s law further limits when police can use deadly force, saying it’s allowed “only when necessary in defense of human life.” Previously, an officer could be justified in shooting someone if doing so was deemed “reasonable” - a standard that many civil rights advocates believed was vague and allowed police to kill with impunity. In both cases, officers who fired the fatal shots are facing criminal charges as a result of a closely watched and contested state law that took effect on Jan. That process is now under way in courts in San Diego and Alameda counties. “We want the officers to be held accountable.” “I want people to understand that our children are important to us and that we want justice,” said Kitchen, a retired prison guard. ![]() 12/9/2023 0 Comments Pathological gambling center![]() ![]() An early report 9 of 232 GA attendees noted that early dropout in GA is common, with 48% failing to return within their first three meetings and only 8% achieving at least 1 year of abstinence. Relatively few studies have been conducted on GA as part of recovery, particularly among those who use GA as their primary treatment mode. 8 Family members can attend sister meetings (GamAnon) or in some cases attend open meetings with the affected member. For example, members experienced in managing their own debts may provide aid in setting up debt repayment plans and negotiating with creditors. Senior members provide support for gambling recovery (eg, dealing with cravings, slips, acknowledging progress) and the negative consequences of gambling. ![]() Social support from peers and family members is an integral part of the organization. 8 For this reason, any beliefs gamblers may hold about their ability to control their gambling now or in the future are actively discouraged. Like its sister programs, GA has adopted the disease model and views disordered gambling as a lifelong affliction that can be controlled via gambling abstinence, but not cured. GA, similar to the Alcoholics Anonymous 12-step programs upon which it is modeled, strongly advocates complete abstinence from gambling. Intervention in this group may prevent escalation of gambling to the more severe form of the disorder. Subthreshold gamblers, who meet fewer criteria, are often referred to as problem gamblers they can experience some negative consequences related to their gambling and may be at risk for escalation of their gambling problems. ![]() Diagnosis of gambling disorder is met when four or more of these criteria are present in a 12-month period. In the present article, the term “pathological gambling” will be used as described in the prior edition (Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition ).ĭSM-5 criteria for gambling disorder remain largely intact from the prior edition and include: 1) needing to wager larger amounts 2) experiencing restlessness or irritability when restricting gambling 3) repeated inability to control or stop gambling 4) preoccupation with gambling 5) gambling in response to negative affect 6) gambling to recoup losses 7) lying about gambling 8) jeopardizing relationships, work, or educational opportunities due to gambling and 9) relying on financial bailouts from others to relieve financial pressures related to gambling. In addition, its name was changed from pathological gambling to gambling disorder. In the Diagnostic and Statistical Manual (DSM) of Mental Disorders, Fifth Edition (DSM-5), 1 gambling disorder was relocated from the impulse control disorder chapter to the newly-expanded chapter for substance-related and addictive disorders. Overall, several treatments have been developed for gambling disorder and results are promising, but variability in findings suggests a need for further systematic evaluation.ĭisordered gambling is a maladaptive pattern of wagering that persists despite negative consequences in major areas of functioning such as finances, relationships, and psychological health. Of more extensive therapies, no consistent benefit of one approach emerges, although cognitive–behavioral interventions have been most often applied. ![]() Brief and motivational approaches similarly may extend treatment options to more gamblers, namely at-risk and problem gamblers and those not seeking treatment. These self-directed options reduce the barriers associated with treatment-seeking, and may reach a wider range of gamblers than professionally delivered treatments alone. Self-directed interventions appear to benefit some gamblers however, the involvement of therapist support, either in person or by telephone, may bolster these effects and such support need not be extensive. Involvement in peer support programs seems to be optimal when combined with professional treatment however, engagement and retention in peer support is limited. Several treatment options for gamblers have been explored, ranging from self-help and peer support, to brief and motivational interventions, to more intensive therapy approaches. This review discusses the research evidence for psychological treatment of gambling disorder. ![]() 12/9/2023 0 Comments Expertgps crack![]() No other software makes it this easy to add your own data to maps and aerial photos. I hope you enjoy this full-featured ExpertGPS trial. Whether you're discovering a new trail or scouting a 400 acre parcel, ExpertGPS gives you the maps and tools you need to get the job done. GPS Trackmaker works differently enough and also. Play tracks and discovery playlists tagged expertgps on SoundCloud desktop. Torrent Expertgps EasyGPS a handy and easy-to-use. Disconnect your laptop and head out on a trip, and all of your data is available. ExpertGPS 8.10 Crack + Serial Number Free Download 2022xpertGPS 8.10 Crack might be a pleasant application. ExpertGPS Pro 7.13 Crack With KeygenLatest Version Free Download 2020 ExpertGPS Pro Crack is a powerful software utility designed to help clients view and modify waypoints, routes, and GPS tracks.An easy-to-use application that comes with the help of a wide range of card codecs. With ExpertGPS, you'll be able to calculate acreage, measure distance, elevation and grade.ĮxpertGPS downloads aerial photos and topo maps for any location in the US, saving the maps to your hard drive. You can reproject data in any geographic format (lat-lon, UTM, US State Plane) and change datums instantly. You'll be able to convert any GPS, GIS, or CAD data to or from GPX, Google Earth KML or KMZ, Excel CSV or TXT, SHP shapefiles, or AutoCAD DXF drawings in two clicks. View your GPS tracklog over an aerial photo to see exactly where you went.ĮxpertGPS handles all your mapping and data-conversion jobs. Plan your next outdoor adventure over scanned USGS topo maps and send a route directly to your GPS receiver to guide you in the field. Expertgps Serial Key Software Serial Key Generator is application specially designed to help you protect your applications by serial key registration. View GPS waypoints and tracklogs from any handheld GPS receiver over aerial photos and US topographic maps. More, An easy-to-use this app that comes with a wide range of card codecs. Описание (ru) ExpertGPS картографическое программное решение для обладателей навигаторов Garmin, Magellan и Lowrance. Our flagship product, ExpertGPS, allows anyone to view data from a Garmin, Magellan or Lowrance GPS receiver over seamless topo maps and aerial photography. ![]() 12/8/2023 0 Comments Slither io unblocked online![]() Fixed key strokes not getting registered.+500 is now only shown when you are the one who killed a player.Fixed players not having the same color as their land.Made the spawn algorithm a bit less accurate when a lot of players are spawning at once, this was causing a lot of lag.Changed the limit of amount of players per server to about 70, the map is now 500x500 in size.Changed the port of the team server, if you weren't able to connect to the team server first you might be now.There are also only two lobbies per server now, which should also decrease the amount of lag. Player count is now between 60 and 90 players per lobby. Changed the map size back to 600圆00 to lower the chance of spawning in someones land.Fixed two players being able to kill the same player at once.Fixed dying after having just killed another player.wext mobile update will have support for teams :) Opening a #team url or a url with an #animal in it now asks if you want to open the app on mobile devices.Fixed not being able to connect to a team server when starting a game too quickly after creating a team.Removed the letters L and i from team urls because they look too much like each other.Holding an arrow key while pressing another arrow key now remembers your direction.If you're the creator of the game you'll get fancy goggles.Added an ugly mode toggle to the home screen.When opening a team url on a mobile browser, the page will ask to redirect to the app since the app now has team mode support.You can now toggle leaderboard visibility using the 'o' key.Fixed an issue that would break servers entirely, causing a 'Couldn't connect' error to appear whenever you tried to join.Fixed #animal names of servers changing every minute.Fixed an issue where other players would have a ghost trail while they were in their own land.Fixed keys getting stuck when holding them and clicking away the focus from the browser window.Fixed being able to kill yourself by pausing and then moving in the opposite direcion (making a 180 degree turn).Fixed 'o' key toggling leaderboard even in the login screen.This way desktop players are able to play cross platform with mobile players. If you open a team url in your browser, it will automatically ask you to open the app. Push the left mouse key dash.The latest version of the mobile app (1.4) has support for the teams gamemode. * LeaderboardsControlsUse your mouse to go around. io games for lots more multiplayer entertainment!ĭeveloperSlither.io originated by Steve Howse. Don’t forget to examine Agar.io or any other. Slither.io unblocked is the favorite game of several on line gamers, and it’s really sure to come to be one of your go-to games, also. Trick other people and consume all of them, and try to avoid being cut off and bursting into shining particles yourself. Eat whenever you can easily to cultivate and become the largest worm. Twirl around and steer clear of various other people. You can view your overall score and ranking when you look at the bottom left place regarding the screen, as the server-wide leaderboard is found in the upper correct part. Watch out for other people! Touching their body will destroy both you and change you into a delicious meal! They’re going to attempt to fool you into crashing to their lengthy, snake-like body but you can also let them have a taste of their own medicine and rapidly slither right in front side of these! Multiplayer games have a leaderboard system, and Slither.io is not any exception. ![]() Might develop rapidly while you consume radiant orbs, but. When you start the overall game, you’ll be just the smallest small sliver of a snake. There are lots of colorful options to choose from. If you need, you are able to transform and personalize the look of your worm, too. Prior to starting the game, give your personality a name. You merely need to use your mouse to visit across the chart. As opposed to these types of intense game play knowledge, Slither.io’s controls are pretty easy. Hungry players are hiding in almost every part. Risk can be as normal due to the fact wind in the world of Slitherio. In Slither.io, just the many mindful players survive to be the biggest! Competition is constant! Everybody will attempt to eat you, and no one trusts any person! Eliminate other people utilizing razor-sharp turns and wise maneuvers, and attempt to collect as numerous radiant orbs as you possibly can! Slither your path in to the world of glowing orbs, worms, and insatiable appetite. io games, and offers hours of enjoyable featuring its leaderboards and action-packed game play. Slither.io unblocked is one of the most popular web multiplayer. ![]() 12/8/2023 0 Comments Hype video maker![]() A place where you can "fish" and take advantage of people.A complete substitute for tech support - There's no problem asking about common errors or problems, workarounds for glitches, annoying limitations, but at some point, especially with some of the less commonly used tools, like PowerDirector and Filmora, you may wish to contact the developer's tech support services as well as posting here.There are other subreddits where that is more appropriate, like /r/Videos, /r/YouTube_startups, /r/SmallYTChannel, and /r/YouTubers even has monthly show-off threads. A place to show off your work - Feel free to ask for some constructive criticism in our feedback thread, but this is not a place to advertise your YouTube channel. ![]()
12/8/2023 0 Comments War thunder account login![]() 102 A business operated at 100% of capacity during its first month, with the following results: Sales (112 units) $448,000 P.98 Newton's _ law explains why my hands hurt when I clap loudly.115 All calculations need the counting processand Free Body Diagram.113 In regard to the E2 mechanism, rank the leaving groups in order of increasing reaction rate.Step 7: If you are still having issues after performing all the steps here, please contact us! We will be happy to help as soon as possible. We will need to help recover the forgotten password here. Step 6: If VPN is disabled and you are still unable to log in, it might be because of forgetting the password. Some sites may restrict access for certain countries or IP addresses, so they can prevent users from streaming and downloading content that is not allowed in their region. Step 5: Remember to turn off your Virtual Private Network before you do anything else. Clear instructions on how to do this in Chrome or Firefox are included (links provided). Step 4: If the steps above haven't worked for you, try clearing your browser's cache and cookies. ![]() If you can see the password, use it if no one else is around because people might be watching over your shoulder and trying to steal your passwords. Step 2: Type your AmazonSecure login correctly. ![]() If there are any issues with the connection, try checking your router and restarting it before proceeding further. Step 1: Ensure you have a good internet connection. If you are having problems with your account, there is an easy troubleshooting guide to help fix the issue. ![]() ![]() Then, go to the Photo or Edit option (according to the Lightroom version you are working on). ![]() All you have to do is select your stack and the photos you want to add to that stack in the Library module. Add, Delete, Rearrange, Split Stack & Unstack ImagesĪdding and deleting images from a stack is easy. The shortcut key to toggle between expanding and collapsing an image stack is ‘S’. Right-clicking on a photo in a stack will also open stacking options for you. In this menu, you will have the option to expand or collapse all the image stacks in your catalog. Then, from the Stacking option, you can choose to Expand Stack or Collapse Stack. In Lightroom CC, you will have to go to the Edit option. The other way to expand or collapse image stacks is by highlighting the top image and then going to the Photo option in Lightroom Classic. If you want to collapse the stack again, you will have to click on the number in the first photo. The first number denotes the particular image’s position in the stack, while the second number is the total number of photos. You will see two numbers mentioned on every thumbnail. When you click on the number mentioned on the thumbnail, the stack will expand to show all the images. How to Expand or Collapse Image Stacks in LightroomĪll Image Courtesy of ShootDotEdit Customer a stack simply means displaying all the photos from a stack, and collapsing it does just the opposite. You will be able to group and ungroup photos and set one as your stack’s cover image. Also, Lightroom CC will provide you with fewer image grouping options. If you are editing in Lightroom CC, you will be able to access the image stacking options from the Edit dropdown menu. Go to the Interface tab next and tick the Show Stack Counts box. If you are working in Lightroom Classic and do not see a number on the top picture, select Preferences from the Lightroom Classic dropdown menu. This number indicates the number of images that have been stacked in that group. You will now see one thumbnail with a number mentioned in the upper left corner. After this, the look of your Filmstrip will change. You can also use the shortcut Command + G on Mac and Ctrl + G on Windows. To begin stacking photos, click on the Photo dropdown menu and select the Stacking option. Now, to select photos, you’d need to hold down the shift key to select multiple pictures in a row or use the Command key on Mac or Control key on Windows to click and choose individual images. In the Filmstrip view, numbers will help you identify images that are a part of a stack, but in the Grid view, you will only be able to see the top photo without any number or sign on it. You could also press F6 to get to the Filmstrip View. From the menu, select Panels and then Show Filmstrip. If you want to stack images in the Filmstrip View, you can select the option at the bottom of your screen or go to the Windows dropdown menu. The Grid View is going to be your Default view if you haven’t modified it. You will be able to do this in the Library or the Develop module. You'll notice the same choices you're used to before Additionally, we can decide what happens here.All Image Courtesy of ShootDotEdit Customer stack photos in Lightroom Classic, select the images you need to stack first. ![]() and that they light up for a panoramic image. And if you had an HDR panorama, you'd have to first move all the panoramas and merge 'em together, HDR panorama, and Lightroom will analyze. You originally could merge and HDR, or merge a panorama. This HDR panorama option wasn't originally available when these features first shipped in Lightroom. And you'll notice an option called HDR panorama. What we'll do here is select all of the images to start. on a moving bridge with a lot of people, and clouds and sky and other areas, I really wanted to keep this moving along. These three pictures together cover the scene nicely. ![]() ![]() the base exposure plus an underexposed image, and an overexposed image. In this case we've got two HDR panoramas loaded, and what we're going to do is put these pieces together. ![]() |
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